Gifani, A., Marzban, R., Dezianian, A. (2015). Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation. , 3(1), 1-8. doi: 10.22092/bcpp.2015.102970
Aida Gifani; Rasoul Marzban; Ahmad Dezianian. "Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation". , 3, 1, 2015, 1-8. doi: 10.22092/bcpp.2015.102970
Gifani, A., Marzban, R., Dezianian, A. (2015). 'Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation', , 3(1), pp. 1-8. doi: 10.22092/bcpp.2015.102970
Gifani, A., Marzban, R., Dezianian, A. Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation. , 2015; 3(1): 1-8. doi: 10.22092/bcpp.2015.102970

Evaluation some polymer combinations in improvement of HaNPV stability against ultraviolet radiation

مهار زیستی در گیاه‌پزشکی
Article 1, Volume 3, Issue 1, April 2015, Pages 1-8    PDF (635.18 K)
Document Type: Research Paper
DOI: 10.22092/bcpp.2015.102970
Authors
Aida Gifani; Rasoul Marzban* ; Ahmad Dezianian
Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract
The current project was carried out to investigate the role of microencapsulation and influence of different polymers on the protection of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) against ultraviolet radiation.  For this purpose, microencapsulated formulation of HaNPV was produced by emulsion technique using three natural polymers including sodium alginate, gelatin, and starch individually in and combined with each other. After exposing to UV irradiation for 5 and 24 hours, the stability and viral activity of the obtained formulations were evaluated by bioassay test on H. armigera larvae. However, the mortality rate for the non-microencapsulated virus suspension, declined from 100% before UV radiation to 46.66% after 24 hours exposing to the UVA radiation. Before UV radiation, each of the sodium alginate and starch microencapsulated formulations separately, not only could provide a proper coating for HaNPV virus, but also decreased the viral activity. Whereas the combination of these two polymers together had a synergistic effect and resulted in an increased mortality. After 5 and 24 hours exposing to UV, their combination was significantly different from the non-microencapsulated formulation (Duncan test, P<0.05). After 5 and 24 hours exposing to UV, the difference of gelatin and its combinations with the non-microencapsulated formulation was significant.

 
Keywords
biopesticide; HaNPV; UV radiation; Microencapsulation; polymer
References

Acosta, S., Jiménez, A., Chiralt, A., González- Martinez, C., & Cháfer, M. 2013. Mechanical, barrier and microstructural properties of films based on cassava starch gelatin blends: effect of aging and lipid addition. Inside Food Symposium, 9-12 April, Leuven, Belgium.

Anonymous, 2000. UVA Technology Planning and Management Corporation. FINAL report on carcinogens: background document for broad-spectrum ultraviolet radiation and UVA, UVB, and UVC. Meeting of the NTP board of scientific counselors report on carcinogens subcommittee, http://ntp.niehs.nih.gov/ntp/newhomeroc/roc10/uv_no_appendices_508.pdf.

Bouwer, G. & Avyidi, D. 2006. Application of the droplet feeding assay method to two economically important African lepidopteran pests: short communication. African Entomology, 14: 195-198.

Dezianian, A. 2011. Biological Control of the Diamondback Moth (plutella xylostella Linnaeus) using plutella xylostella Granulovirus. Thesis Presented to the Senate of University Putra Malaysia in Fulfillment of the Requirement for the degree of Doctor of Philosophy.

Filho, A.B., Alves, S.B., Augusto, N.T. & Pereira, R.M. 2001. Stability and persistence of two formulations containing Anticarsia gemmatalis Nuclear Polyhedrovirus (AgMNPV). Neotropical Entomology, 30: 411-416.

Gifani, A., Taghizadeh, M., Seifkordi, A.A. & Ardjmand, M. 2009. Preparation and investigation the release behaviour of wax microspheres loaded with salicylic acid. Journal of Microencapsulation, 26: 485–492.

Ignoffo, C.M. & Garcia, C. 1992. Combinations of environmental factors and simulated sunlight affecting activity of inclusion bodies of Heliothis (Lepidoptera: Noctuidae) nucleopolyhedrosis virus. Journal of Environmental Entomology, 21: 210-213.

Junxin, Y., Defu, C. & Jia, Y. 2011. Encapsulated Bacillus thuringiensis powders by internal gelation of  alginate microspheres for stability. Advanced Materials Research, 183: 272-277.

Kailasapathy, K. 2002. Microencapsulation of probiotic bacteria: Technology and potential applications. Current Issues in Intestinal Microbiology, 3: 39-48.

Khorramvatan, S., Marzban, R., Ardjmand, M., Safekordi, A.A. & Askary, H. 2013. The effect of polymers on the stability of microencapsulated formulations of Bacillus thuringiensis subsp. kurstaki (Bt-KD2) after exposure to Ultra Violet Radiation. Biocontrol Science and Technology, 24: 462-472.

Liu, X.X., Wang, Y.F., Zhang, N.Z., Shank, R.A., Liu, H.S., Tong, Z., Chen, L. & Yu, L. 2014. Morphology and phase composition of gelatin-starch blends. Chinese Journal of Polymer Science, 32: 108−114.

Masuda, M. 2011. Microencapsulation of Pesticides for Controlling Release from Coatings. Department of Chemical and Biological Engineering Chalmers University of Technology, Göteborg, Sweden.

Puttipipatkhachorn, S., Pongjanyakul, T. & Priprem, A. 2005. Molecular interaction in alginate beads reinforced with sodium starch glycolate or magnesium aluminum silicate, and their physical characteristics. International Journal of Pharmaceutics, 293: 51–62.

Rodriguez, V.A., Belaich, M.N. & Ghiringhelli, P.D. 2012. Baculoviruses: Members of integrated pest management strategies. http://cdn.intechopen.com/pdfs-wm/29617.pdf.

Sajap, A.S., Bakir, M.A., Kadir, H.A. & Samad, N.A. 2007. Effect of pH, rearing temperature and sunlight of infectivity of Malayasian isolate of nucleopolyhedrovirus tolarvae of Spodoptera litura (Lepidoptera: Noctuidae). International Journal of Tropical Insect Science, 27: 108–113.

Sultana, K., Godward, G., Reynolds, N., Arumugaswamy, R., Peiris, P., & Kailasapathy, K. 2000. Encapsulation of probiotic bacteria with alginate–starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. International Journal of Food Microbiology, 62: 47–55.

Tamez-Guerra, P., Michael, R.M., Behle, R.W., Hamm, J.J., Sumner, H.R. & Shasha, B.S. 2000. Sunlight persistence and rainfastness of spray-dried formulations of Baculovirus isolated from Anagrapha falcifera (Lepidoptera: Noctuidae). Journal of Economic Entomology, 93: 210-218.

Teakle, R.E., & Jensen, J.M. 1991. Heliothis armigera, pp. 313-332. In: Singh, p. & Moore, R.F. (eds.), Hand Book of Insect Rearing. Elsevier, Amsterdam.

Villamizar, L., Barrera, G., Cotes, A.M. & Martinez, F. 2010. Eudragit S100 microparticles containing Spodoptera frugiperda nucleopolyehedrovirus: Physicochemical characterization, photostability and in vitro virus release. Journal of Microencapsulation, 27: 314–324.

Wakil, W., Ghazanfar, M.U., Nasir, F., Qayyum, M.A. & Tahir, M. 2012. Insecticidal efficacy of Azadirachta indica nucleopolyhedrovirus and chlorantraniliprole singly or combined against field populations of Helicoverpa armigera Hubner (Lepideptora: Noctuidae). Chilean Journal of Agricultural Research, 72.

Winder, R.S., Wheeler, J.J., Conder, N., Otvos, I.S., Nevill, R., & Duan, L. 2003. Microencapsulation: a strategy for formulation of inoculum, Biocontrol Science and Technology, 13: 155-169.

Yang, W., He, K., Zhang, J. & Guo, S. 2012. pH-Controlled Bacillus thuringiensis Cry1Ac protoxin loading and release from polyelectrolyte microcapsules. PLoS ONE 7(9): e45233.

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