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تغییر برخی ویژگیهای بیوشیمیایی گیاه دارویی سرخارگل (Echinacea purpurea (L.) Moench) در پاسخ به تاریخ کشت و دوره غرقاب خاک | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 12، دوره 31، شماره 2 - شماره پیاپی 70، خرداد 1394، صفحه 315-331 اصل مقاله (320.82 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2015.101483 | ||
| نویسندگان | ||
| سمانه اسدی صنم1؛ محسن زواره* 2؛ همتاله پیردشتی3؛ فاطمه سفیدکن4؛ قربانعلی نعمتزاده5؛ ابوذر هاشمپور6 | ||
| 1دانشجوی دکترا، گروه زراعت، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| 2استادیار، گروه زراعت، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| 3دانشیار، گروه زراعت و اصلاح نباتات، پژوهشکده ژنتیک و زیستفناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری | ||
| 4استاد، بخش تحقیقات گیاهان دارویی و محصولات فرعی، مؤسسه تحقیقات جنگلها و مراتع کشور | ||
| 5استاد، گروه زراعت و اصلاح نباتات، پژوهشکده ژنتیک و زیستفناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری | ||
| 6دانشجوی دکترا، گروه باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان | ||
| چکیده | ||
| با هدف بررسی اثر تاریخ کشت و دوره غرقاب خاک بر ظرفیت آنتیاکسیدانی گیاه دارویی سرخارگل (Echinacea purpurea (L.) Moench)، آزمایشی بهصورت کرتهای خرد شده در قالب طرح بلوکهای کامل تصادفی با سه تکرار در مزرعه پژوهشی پژوهشکده ژنتیک و زیستفناوری طبرستان-دانشگاه علوم کشاورزی و منابع طبیعی ساری در سال 1391 اجرا شد. تیمارهای آزمایشی شامل سه تاریخ کشت (10 تیر، 9 مرداد و 8 شهریور 1391) و سه دوره غرقاب (بدون غرقاب بهعنوان شاهد، 3 روز غرقاب و 5 روز غرقاب) بودند که بهترتیب در کرتهای اصلی و فرعی در نظر گرفته شدند. پس از پایان مدت غرقاب، مقدار مالوندیآلدهید (MDA) و اکسیداسیون پروتئین برگها، فعالیت آنزیمهای سوپراکسیددیسموتاز (SOD)، آسکوربات پراکسیداز (APX)، پراکسیداز (POD) و کاتالاز (CAT)، میزان فنل و فلاونوئید کل و درصد بازدارندگی رادیکال آزاد DPPH اندازهگیری شدند. نتایج آزمایش افزایش معنیدار مقدار MDA را در برگ سرخارگل نشان داد که بیشترین مقدار آن در غرقاب 5 روزه و در تاریخ کشت 8 شهریور بدست آمد. در این تاریخ کشت، پروتئین کل 5 روز پس از غرقاب نسبت به شاهد 90% کاهش یافت. بیشترین فعالیت آنزیمهای آنتیاکسیدانی SOD و APX در سرخارگلهای کشت شده در تاریخ 10 تیر و در 3 روز پس از غرقاب بدست آمد، در حالیکه بیشترین فعالیت آنزیم POD و CAT مربوط به سرخارگلهای نشاء شده در تاریخ 9 مرداد بود. غرقاب 5 روزه سرخارگلها، میزان فنل و فلاونوئید کل گیاهان نشاء شده در تاریخ 10 تیر را افزایش داد. بیشترین درصد بازدارندگی رادیکال آزاد DPPH (1/79%)، در 5 روز پس از غرقاب و در تاریخ کشت 8 شهریور بدست آمد. بهطور کلی، با توجه به یافتههای این آزمایش میتوان گفت که گیاه سرخارگل به تنش غرقاب تحمل نسبتاً خوبی دارد. | ||
| کلیدواژهها | ||
| آنزیمهای آنتیاکسیدان؛ پروتئین؛ سرخارگل (Echinacea purpurea (L.) Moench)؛ فلاونوئید؛ مالوندیآلدهید | ||
| عنوان مقاله [English] | ||
| Changes in some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) medicinal plant in response to planting date and soil flooding duration | ||
| نویسندگان [English] | ||
| S. Asadi Sanam1؛ M. Zavareh2؛ H. Pirdashti3؛ F. Sefidkon4؛ Gh.A. Nematzadeh5؛ A. Hashempour6 | ||
| 1Ph.D. Student, Department of Agronomy, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Department of Agronomy, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 3Department of Agronomy and Plant Breeding, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran | ||
| 4Medicinal Plants Research Division, Research Institute of Forests and Rangelands, Tehran, Iran | ||
| 5Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 6Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| This research was aimed to investigate the effect of planting date and soil flooding duration on some biochemical characteristics of purple coneflower (Echinacea purpurea (L.) Moench) in Sari region. The study was conducted in a RCBD based split plot with three replications in the Research Farm of the Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, in 2012. Experimental treatments included three planting dates (June 30, July 30 and August 29) and three soil flooding durations (without flooding as control, three and five-day flooding) which were considered as main and sub-plots, respectively. Malondialdehyde (MDA), total phenols and flavonoids contents, protein oxidation of the leaves, enzyme activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD) and catalase (CAT), as well as percentage of DPPH free radical inhibition were determined. Results of this experiment showed a significant increment of malondialdehyde (MDA) content in purple coneflower leaf with the highest level in five-day flooding duration and in August 29 planting date. The highest decrease in total protein was found in the same planting data and flooding duration with 90 % decline than control.The highest activity of antioxidant enzymes of superoxide dismutase (SOD) and ascorbate peroxidase (APX) was recorded in the purple coneflowers leaves, cultivated in June 30, and flooded for three days, while the highest activity of peroxidase (POD) and catalase (CAT) were observed in plants cultivated in July 30. Five-day soil flooding markedly increased the total phenols and flavonoids content just in plants transplanted in June 30. In addition, the highest percentage of DPPH free radical inhibition was measured in plants subjected to continues five-day soil flooding and cultivated in August 29. In conclusion, it seems that the coneflower plant relatively showed a good tolerance to flooding stress. | ||
| کلیدواژهها [English] | ||
| Antioxidant enzymes, protein, purple coneflower (Echinacea purpurea (L.) Moench), flavonoid, malondialdehyde | ||
| مراجع | ||
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