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تاثیر سطوح متفاوت عصاره تفاله انگور بر قابلیت هضم روده‌ای گاو شیری به روش برون تنی | ||
| علوم دامی | ||
| مقاله 2، دوره 28، شماره 106، خرداد 1394، صفحه 13-28 اصل مقاله (1.22 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2015.101344 | ||
| نویسندگان | ||
| محمد جواد ابرقوئی1؛ یوسف روزبهان* 2 | ||
| 1دانش آموخته دکتری تغذیه دام دانشکده کشاورزی دانشگاه تربیت مدرس | ||
| 2عضو هیئت علمی گروه علوم دامی دانشکده کشاورزی دانشگاه تربیت مدرس | ||
| چکیده | ||
| این تحقیق به منظور بررسی سطوح مختلف عصاره تفاله انگور بر تجزیهپذیری و قابلیتهضم جیره معمول گاو شیری با استفاده از روش تولید گاز دو مرحلهای تغییر‌یافته، انجام پذیرفت. مقادیر اسیدهای چرب فرار، تولید آمونیاک و جمعیت پروتوزوآ تعیین گردیدند. شش سطح عصاره مورد استفاده قرار گرفت که شامل GE0: جیره پایه بدون عصاره، GE1، GE2، GE3، GE4، GE5 به ترتیب 12، 24، 36، 48 و 60 میکرولیتر عصاره به ازای 50 میلیلیتر محیط تلقیح بود. تجزیهپذیری شکمبه‌ای ماده‌خشک تیمارهای GE3، GE4 و GE5 در مقایسه با GE0 کاهش یافت، ولی تجزیهپذیری شکمبه‌ای پروتئین‌خام در کل تیمارهای حاوی عصاره نسبت به تیمار شاهد کاهش نشان داد. قابلیتهضم ماده‌خشک و پروتئین‌خام در تیمارهای GE4 و GE5 در مقایسه با تیمار GE0 کاهش یافت. استفاده از عصاره، مقدار پروتئین عبوری و قابلیتهضم پروتئین‌خام عبوری در محلول پپسین- اسید هیدروکلریدریک را در مقایسه با تیمار شاهد، افزایش داد. جمعیت باکتری‌های پروتئولیتیک در تیمارهای GE4 و GE5 نسبت به تیمار شاهد کاهش یافت. مقدار کل اسیدهای چرب فرار، استات و نسبت استات به پروپیونات در تیمارهای GE3، GE4 و GE5 در مقایسه با تیمار شاهد کمتر بود. افزودن عصاره باعث کاهش مقدار تولید آمونیاک، جمعیت کل پروتوزوآ، زیرخانواده انتودینینه1 و جنس داسی‌تریچا3 شد. جنس ایزوتریچا2، زیرخانواده دیپلودینینه4 و زیرخانواده افریوسکالسینه5 در زمان‌های 12 و 24 ساعت بعد از گرمخانهگذاری با افزودن عصاره کاهش یافت. در کل، استفاده از عصاره تفاله انگور، مقدار آمونیاک، جمعیت پروتوزوآ و تجزیه پروتئین را کاهش و قابلیت هضم‌پروتئین عبوری از شکمبه را افزایش داد | ||
| کلیدواژه‌ها | ||
| گاو شیری؛ تفاله انگور؛ آزمون تولید گاز؛ قابلیت هضم؛ پروتوزوآ؛ برون تنی | ||
| عنوان مقاله [English] | ||
| The effects of several levels of grape pomace extract on in vitro intestinal digestibility of dairy cow | ||
| نویسندگان [English] | ||
| Mohamad javad Abarghoyi1؛ Yousef Rouzbehan2 | ||
| چکیده [English] | ||
| The experiment was carried out to study the effect of different doses of grape pomace extract (GE) on ruminal degradation and post-ruminal digestion of basal diet in dairy cow using a modified two-step in vitro method. The VFA, ammonia production and protozoa population determined. Six levels of extract were used: GE0: basal diet, GE1, GE2, GE3, GE4, GE5 contains 12, 24, 36, 48 and 60 µl extract/50 ml inoculum respectively. Ruminal degradation of DM (rdDM) in GE3, GE4, GE5 was lower comparing to GE0, but ruminal degradation of CP (rdCP) by adding GE was decreased. Digestibility of DM (tdDM) and CP (rdCP) in GE4 and GE5 diets were lower with comparing to GE0. Addition of GE increased the proportion of rumen escape CP (pdBCP) and degradability of bypass CP in HCl/pepsin (dBCP). The population of proteolytic bacteria only declined in GE4 and GE5 diets comparing to GE0. Total VFA, acetate concentrations and acetate propionate ratio were lower in GE3, GE4, GE5 diets compared to GE0. NH3-Nproduction, total number of protozoa, subfamily of Entodiniinae and genus of Dasytricha were decreased as the level of GE increased. With the By addition of GE, genus of Isotricha and subfamilies of Diplodiniinae and Ophrioscolecinae at the 12 and 24 h of incubation was lower than the control. In conclusion, GE supplementation has reduced protozoa population, NH3-N and protein degradation in the rumen and increased degradability of bypass CP | ||
| کلیدواژه‌ها [English] | ||
| Dairy cow, grape pomace, gas test, digestibility, protozoa and in vitro | ||
| مراجع | ||
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