Study of Hg effects on liver tissue in Rutilus rutilus of Caspian sea in vitro | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| Editorial, Volume 24, Issue 3, September 2011, Pages 23-29 PDF (414.64 K) | ||
| DOI: 10.22092/vj.2011.101090 | ||
| Authors | ||
| S Mohamadzade.Baran1; GH.H Vosoghi1; A Mashinchian1; F Abbasi* 2; P GhavamMostafavi1 | ||
| 1Department of Marine Biology, Faculty of Marine Science and Technology, Science and Research Branch Islamic Azad University, Iran. Department of Biology, , Iran | ||
| 2Alzahra University | ||
| Abstract | ||
| A series of specific toxicological effects including bioaccumulation of the pollutant, histological changes and influences on liver tissue were examined in the Roach fish (Rutilus rutilus) after the exposure to graded sublethal concentrations (10 , 30 , 50 μg Hg/L) of mercury chloride (MC). Histopathological changes, such as oedema, vacuolization, pyknotic nucleus, telangiectasis, and degenerative, can clearly be observed in the slices from the liver exposed Roach. A batch of Roach with the mean values of 5 cm in length and 5.5 g in mass were pre-raised in a static system, which was supplied with dechlorinated tap water and constantly aerated. The water qualities were controlled as follows: pH, 7.45; oxygen concentration, 7 mg/L; the water temperature, 21–22 ◦C. All fish were fed twice a day with a commercial ration of biomar. Reagents Appropriate amount of mercury chloride (MC, AR 98%, Merck-Schuchardt) was dissolved in a stock solution of 0.676g HgCl2/L. Based on the 24 h LC50 value (70 ppb) obtained from the acute exposure test, a graded series (10 , 30 , 50 μg Hg/L) of mercury chloride (MC) were administered to Roach fish (Rutilus rutilus) with 48 fish per group. The chemical exposure experiments lasted for 48 , 96 and 144 hours. These designed exposure levels were sublethal, and could induce obvious alteration of tissues abnormalities and disease at the same time. | ||
| References | ||
|
1- فروغی، ر.، اسماعیلی ساری، ع.، قاسم پوری، م. (1385) مقایسه همبستگی طول و وزن با تراکم جیوه در اندام های مختلف ماهی سفید سواحل مرکزی خزر جنوب. دانشگاه تربیت مدرس نور.## 2- یزدانی، ل. (1384) سنجش الودگی جیوه در بافت های ماهی کفال طلایی (Liza auratus) سواحل غربی استان مازندران. پایان نامه کارشناشی ارشد دانشگاه تربیت مدرس.## 3- Agah، H. Leermakers، M. Elskens، M. Fatemi، S.M.R. and Baeyens، W. (2006) Total Mercury and Methylmercury Concentrations in Fish from the Persian Gulf and the Caspian Sea. Water Air Soil Pollution، 181: 95–105pp.##
4- Carvalho S, Lombardi JV, Paiva MJ, de França-Monkolski JG, Ferreira JR. (2006) Bioaccumulation of Mercury in Fish Exposed to Experimentally Contaminated Water and Sediment. Bull. Environ Contam Toxicol. 77: 854–860pp.##
5- Filenko، O.F. Xihua، D. Xulong، C. and Yuqi، Z. (1989) Distribution of mercury in the tissues of carp and its biological effects. Hydrobiol. J، 24 : 64-68pp.##
6-Johnson A.K., Thomas P. and Wilson JR R.R. (1998) Seasonal cycles of gonadal development and plasma sex steroid levels in Epinehelus morio a protogynous grouper in the eastern Gulf of mexico, J. fish Bio. 52, 502 – 518##
7- Liao, Ch. Fu J. Shi J. Zhou Q. Yuan Ch. Jiang Gu. (2006) Methylmercury accumulation، histopathology effects، and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride. Environmental Toxicology and Pharmacology 22: 225–233pp.##
8- Mela، M. ventura D.F. Randi M.A.F. and carvalho C.E.V. (2007) Effects of dietary methlmercury on liver and kidney histology in the neotropical fish Holplias malabaricus. Ecotoxicology and environmental safety. 68 : 426-435pp.##
9- Oliveira Ribeiro, C. A., Torres, R.F. (1995) Acute effects evaluation of inorganic mercury on epidermis of Trichomycterus brasiliensis. Toxicant Environ saf, 32 : 260-266 pp.##
10- Oliveira Ribeiro, C. A. Belger, L. Pelletier, É. and Rouleau C. (2002) Histopathological evidence of organic mercury and methyl mercury toxicity in the arctic charr (Salvelinus alpinus). Environmental Research, 90 : 217-225pp.##
11-Teh, S. J. Adams, S. M. and Hinton D. E. (1997) Histopathologic biomarkers in feral freshwater fish populations exposed to different types of contaminant stress . Aquatic Toxicological, 37:51-70 pp.##
12- Zalups، R.K. (2000) Moleculor interactions with mercury in the kidney. Pharmacological Reviews Vol. 52, No. 1. 113-144pp.## | ||
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