Molecular detection of Ornithobacterium rhinotracheale in broiler chickens with respiratory infection in Shahrekord | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| Editorial, Volume 25, Issue 3, September 2012, Pages 41-44 PDF (370.07 K) | ||
| DOI: 10.22092/vj.2012.101056 | ||
| Authors | ||
| A Zamani Moghaddam1; H Tahmasby* 2; S.H Hashemi Babaheidari3; M Khosravi Farsani3; A Kiani Salmi4 | ||
| 1Associate Professor, Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran | ||
| 2Student, Faculty of Veterinary Medicine and Member of Research Institute of Biotechnology, University of Shahrekord, Shahrekord, Iran | ||
| 3Student, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran | ||
| 4Lab Technician, Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran | ||
| Abstract | ||
| Respiratory disease conditions are continuing to cause heavy economic losses in the poultry industry worldwide. Ornithobacterium rhinotracheale (ORT) has been incriminated as a possible additional causative agent in respiratory disease complex. Although, ORT has been proved to be highly sensitive to different chemical disinfectants, currently, ORT infection appears to have become endemic and can affected every new restocking even in previously cleaned and disinfected houses especially in areas with intensive poultry production as well as in multiple age farms. Considering the importance of what mentioned, present study was conducted to detection of Ornithobacterium rhinotracheale in broiler chickens with respiratory infection from Shahrekord, Iran. Altogether 93 tracheal swab samples collected from 31 broiler chicken flocks with respiratory diseases in Shahrekord, Iran. The samples were plated onto Blood agar containing Gentamicin and 5% defibrinated sheep blood and incubated under 5-7.5% CO2 at 37°C for 48 hours. Suspected colonies were evaluated for detecting ORT by polymerase chain reaction (PCR) method. The rate for respiratory infections with ORT was 19.14% (18 out of 93). Present study suggests ORT can play a significant role in economic losses caused by respiratory diseases in the poultry industry of the region. | ||
| References | ||
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1- اسدپور، ی.، بزرگمهری فرد، م.ح.، پوربخش، س.ع.، بنانی، م.، چرخ کار، س. (1388) تشخیص O. rhinotracheale در گله های مرغ مادر گوشتی استان گیلان به روش PCR. مجله دامپزشکی ایران، 5، 4: 59-63 2- بنانی، م.، پوربخش، س.ع.، ارمی، م.، غلامین، ف.، فاتح منش، م. (1388) شناسایی O. rhinotracheale به روش واکنش زنجیره ای پلیمراز (PCR). مجله تحقیقات دامپزشکی، 64، 1: 41-45 3- جمشیدیان، م.، میاحی، م. (1387) جداسازی O. rhinotracheale (ORT) از ماکیان گوشتی شهرستان اهواز. مجله دامپزشکی ایران، 4، 4: 36-29 4- قائم مقامی، س.ش.، وندیوسفی، ج.، نیرومند، ح.، منصفی، ع.ع.، احمدلو، س. (1386) بررسی شیوع O. rhinotracheale در گله های مرغ گوشتی مبتلا به عوارض تنفسی در استان مرکزی. مجله تحقیقات دامپزشکی، 62، 5: 297-300. 5- Abdul-Aziz, T.A. (1997) Ornithobacterium rhinotracheale developing into a serious infection. World Poultry Misset, 13(8) :47-48 6- Canal, C.W., Leao, J.A., Rocha, S.L., Macagnan, M., Lima-Rosa, C.A., Olivrira, S.D., Back, A. (2005) Isolation and characterization of Ornithobacterium rhinotracheale from chickens in Brazil. Research Veterinary Science, 78(3):225-230 . 7- Chin, R.P., Van Empel, P.C.M., Hafez, H.M. (2003) Ornithobacterium rhinotracheale infection. In: Saif, Y.M., Calnek, B.W., Barnes, H.J., et al. Disease of poultry. Chapter (11th eds). Ames. Iowa State University Press, 683-688. 8- Hafez, M.H. (2002) Diagnosis of Ornithobacterium rhinotracheale. International Journal of Poultry Science, 1(5):114-118 . 9- Hung, A.L. and Alvarado, A. (2001) Phenotypypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from Peru. Avian Disease, 45:999-1005 10- Ozbey, G., Eatas, H.B. and Muz, A. (2005) Random amplified polymorphic DNA (RAPD) Analysis of Ornithobacterium rhinotracheale isolated from chickens in Turkey. Veterinary Medicine-Czech, 50(12):526-530 11- Van empel, P. and Hafez, M.H. (1999) Ornithobacterium rhinotracheale: A review. Avian Pathology, 28:217-227 . 12- Van empel, P. (2002) Ornithobacterium rhinotracheale. In: Jordan, F., Pattison, M., Alexander, D. and Faragher, T . Poultry Diseasees, 5th eds, T. WB Sunders, 138-145. | ||
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