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بررسی تغییرات هیستوپاتولوژیک آبشش ماهی گورخری (Aphanius sophiae) در مسمومیت با آرسنیک و کادمیوم در شرایط آب شور و آب شیرین | ||
| تحقیقات دامپزشکی و فرآورده‌های بیولوژیک | ||
| مقاله 10، دوره 27، شماره 4، دی 1393، صفحه 57-67 اصل مقاله (976.55 K) | ||
| نوع مقاله: مقاله کامل | ||
| شناسه دیجیتال (DOI): 10.22092/vj.2015.100946 | ||
| نویسندگان | ||
| معصومه آریایی1؛ امیرحسین حمیدیان* 1؛ سهیل ایگدری2؛ سهراب اشرفی1 | ||
| 1گروه محیط زیست، پردیس کشاورزی و منابع طبیعی کرج، دانشگاه تهران | ||
| 2گروه شیلات، پردیس کشاورزی و منابع طبیعی کرج، دانشگاه تهران | ||
| چکیده | ||
| آلاینده های اکوسیستم‌های آبی از جمله فلزات سنگین همواره سبب تغییرات آسیب شناختی در ماهیان می شوند. از آنجایی که اثرات آلاینده ها تحت تاثیر ویژگی های شیمیایی آب می باشد، از این رو این پژوهش با هدف مقایسه تاثیرات هیستوپاتولوژیکی دو فلز سنگین Cd و As بر بافت آبشش ماهی بومی Aphanius sophiae انجام شد. در مجموع تعداد 350 قطعه ماهی در طی دو فصل بهار و تابستان 1390 از رودخانه شور اشتهارد نمونه برداری گردیدند. برای آب شور، 7 تیمار حاوی غلظت های 5، 10 و 20 پی پی ام آرسنیک و 5، 10 و 20 پی پی ام کادمیوم به همراه یک تیمار شاهد در آکواریوم های شیشه ای 30 لیتری با سه تکرار طراحی شدند. در هر آکواریوم تعداد 25 ماهی معرفی گردید. طرح مشابه نیز برای آب شیرین با استفاده از آب شهری کلرزدایی شده طراحی و اجرا گردید. پس از 18 روز دوره مواجهه، از هر یک از تیمارهای آب شور و شیرین تعداد 3 ماهی در محلول بوئن تثبیت و سپس آبشش سمت چپ آنها برای بافت شناسی جداسازی گردید. تأثیرات در مراحل اولیه هر دو تیمار مورد بررسی شامل جداشدن لایه اپیتلیال، افزایش تعداد سلول های موکوسی و چسبیدن لاملاهای ثانویه بود. نتایج نشان داد که در تیمار آب شیرین شدت این تغییرات بیشتر از آب شور بود. نکروزه شدن سلول های آبششی، گرزی شدن سر تیغه های آبششی، هیپرپلازی و به‌هم چسبیدن تیغه های اولیه و ثانویه با شدت بیشتری در تیمار آب شیرین مشاهده شد. داده های حاصل براساس آنالیز چند متغیره Two-way NPMANOVA در نرم افزار Past مورد تحلیل قرار گرفتند. با توجه به نتایج این تحقیق می توان نتیجه گیری نمود که کاهش غلظت نمک و به دنبال آن کاهش سختی آب تلفات را به میزان 4/11 برابر افزایش می‌دهد و منجر به افزایش اثرات آسیب های بافتی می گردد. | ||
| عنوان مقاله [English] | ||
| Study of histopathological changes on gill of Aphanius sophiae in arsenic and cadmium toxicity in saltwater and freshwater | ||
| نویسندگان [English] | ||
| M. Ariyaee1؛ A.H. Hamidian1؛ S. Eagderi2؛ S. Asharfi1 | ||
| 1Faculty of Natural Resources, University of Tehran, Karaj. | ||
| 2Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj. | ||
| چکیده [English] | ||
| In aquatic ecosystems, pollutants including heavy metals cause pathological alternations in fishes. As, the impacts of pollutants are affected by chemical properties of water, this study was conducted to compare the histopathological effects of two heavy metals As and Cd on Gill structure of Aphanius sophiae. A total of 350 specimens were collected from the Shoor River of Eshtehard during the spring and summer of 2011. For saltwater experiments seven treatments including 5, 10 and 20 ppm of As and 5, 10 and 20 ppm of Cd plus a control one were prepared in 30-L glass aquariums with three replicates. Twenty five fish specimens were introduced into each aquarium. Similar experiments were designed for freshwater treatments using dechlorinated tap water. After 18 days of exposure, three specimens were sampled from each treatment and fixed into Boein fixative solution and left gill were removed for histological preparation. The observations of prepared histological sections showed alterations including epithelial lifting, increasing goblet cells and fusion of secondary lamellae in both treatments. Result revealed that the severity of these changes in freshwater was more than saltwater treatment. Necrosis, hyperplasia, fusion of first and secondary lamellae had been intensively occurred in freshwater treatments. Data were analyzed by multivariate analysis Two-way NPMANOVA in Past software. It was concluded that decreasing salinity, which led to a decrease in water hardness can proliferate the losses rate of 11/4 times and histopathological effects as a result of As and Cd pollution. | ||
| مراجع | ||
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